nhs depleted of complement protein c8 Search Results


deposit no 203586  (ATCC)
94
ATCC deposit no 203586
Deposit No 203586, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aquapore rp-300 c8 column  (Brownlee Labs)
90
Brownlee Labs aquapore rp-300 c8 column
Aquapore Rp 300 C8 Column, supplied by Brownlee Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c8 column  (NovaBioAssays LLC)
90
NovaBioAssays LLC c8 column
C8 Column, supplied by NovaBioAssays LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti s6  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology anti s6
Anti S6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal antibody against cdk5  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology rabbit polyclonal antibody against cdk5
<t>Cdk5</t> kinase activity is increased in primary cortical neurons grown on myelin extracted from the spinal cord of exercised animals. (A) Primary cortical neurons were grown for 24 h on PDL or on increasing concentration (0.5–2 μg) of myelin extracted from either sedentary (sed-myelin) or exercised animals (ex-myelin). Cdk5-associated kinase activity was determined after immunoprecipitation with anti-cdk5 antibodies and by using histone H1 as a substrate. Panel A shows a representative experiment. (B) Quantification of the effect of 1 μg myelin on Histone H1-associated cdk5 kinase activity in cortical neurons. Data were obtained by PhosphorImager analysis of the auto-radiographs and are expressed as percentage of cdk5 activity found in neuronal cells grown on PDL. Average of four independent experiment ± SEM is shown, *P < 0.05 vs. PDL cultured neurons (Friedman Test followed by Dunn's Multiple Comparison Test). (C, D) Cortical neurons plated for 24 h on exercise-myelin display longer processes compared with cells plated on sedentary-myelin. Pictures of live neuronal cells were taken on a Zeiss inverted microscope (Scale bar = 50 μm)
Rabbit Polyclonal Antibody Against Cdk5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal antibody against cdk5 - by Bioz Stars, 2026-10
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caspase 8  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology caspase 8
<t>Cdk5</t> kinase activity is increased in primary cortical neurons grown on myelin extracted from the spinal cord of exercised animals. (A) Primary cortical neurons were grown for 24 h on PDL or on increasing concentration (0.5–2 μg) of myelin extracted from either sedentary (sed-myelin) or exercised animals (ex-myelin). Cdk5-associated kinase activity was determined after immunoprecipitation with anti-cdk5 antibodies and by using histone H1 as a substrate. Panel A shows a representative experiment. (B) Quantification of the effect of 1 μg myelin on Histone H1-associated cdk5 kinase activity in cortical neurons. Data were obtained by PhosphorImager analysis of the auto-radiographs and are expressed as percentage of cdk5 activity found in neuronal cells grown on PDL. Average of four independent experiment ± SEM is shown, *P < 0.05 vs. PDL cultured neurons (Friedman Test followed by Dunn's Multiple Comparison Test). (C, D) Cortical neurons plated for 24 h on exercise-myelin display longer processes compared with cells plated on sedentary-myelin. Pictures of live neuronal cells were taken on a Zeiss inverted microscope (Scale bar = 50 μm)
Caspase 8, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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automated detection of the point mutation in the factor v gene  (Chemie GmbH)
90
Chemie GmbH automated detection of the point mutation in the factor v gene
<t>Cdk5</t> kinase activity is increased in primary cortical neurons grown on myelin extracted from the spinal cord of exercised animals. (A) Primary cortical neurons were grown for 24 h on PDL or on increasing concentration (0.5–2 μg) of myelin extracted from either sedentary (sed-myelin) or exercised animals (ex-myelin). Cdk5-associated kinase activity was determined after immunoprecipitation with anti-cdk5 antibodies and by using histone H1 as a substrate. Panel A shows a representative experiment. (B) Quantification of the effect of 1 μg myelin on Histone H1-associated cdk5 kinase activity in cortical neurons. Data were obtained by PhosphorImager analysis of the auto-radiographs and are expressed as percentage of cdk5 activity found in neuronal cells grown on PDL. Average of four independent experiment ± SEM is shown, *P < 0.05 vs. PDL cultured neurons (Friedman Test followed by Dunn's Multiple Comparison Test). (C, D) Cortical neurons plated for 24 h on exercise-myelin display longer processes compared with cells plated on sedentary-myelin. Pictures of live neuronal cells were taken on a Zeiss inverted microscope (Scale bar = 50 μm)
Automated Detection Of The Point Mutation In The Factor V Gene, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stanford pan facility n a  (Chem Impex International)
95
Chem Impex International stanford pan facility n a
<t>Cdk5</t> kinase activity is increased in primary cortical neurons grown on myelin extracted from the spinal cord of exercised animals. (A) Primary cortical neurons were grown for 24 h on PDL or on increasing concentration (0.5–2 μg) of myelin extracted from either sedentary (sed-myelin) or exercised animals (ex-myelin). Cdk5-associated kinase activity was determined after immunoprecipitation with anti-cdk5 antibodies and by using histone H1 as a substrate. Panel A shows a representative experiment. (B) Quantification of the effect of 1 μg myelin on Histone H1-associated cdk5 kinase activity in cortical neurons. Data were obtained by PhosphorImager analysis of the auto-radiographs and are expressed as percentage of cdk5 activity found in neuronal cells grown on PDL. Average of four independent experiment ± SEM is shown, *P < 0.05 vs. PDL cultured neurons (Friedman Test followed by Dunn's Multiple Comparison Test). (C, D) Cortical neurons plated for 24 h on exercise-myelin display longer processes compared with cells plated on sedentary-myelin. Pictures of live neuronal cells were taken on a Zeiss inverted microscope (Scale bar = 50 μm)
Stanford Pan Facility N A, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stanford pan facility n a - by Bioz Stars, 2026-10
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mini protean tgx precast protein gel  (Bio-Rad)
99
Bio-Rad mini protean tgx precast protein gel
<t>Cdk5</t> kinase activity is increased in primary cortical neurons grown on myelin extracted from the spinal cord of exercised animals. (A) Primary cortical neurons were grown for 24 h on PDL or on increasing concentration (0.5–2 μg) of myelin extracted from either sedentary (sed-myelin) or exercised animals (ex-myelin). Cdk5-associated kinase activity was determined after immunoprecipitation with anti-cdk5 antibodies and by using histone H1 as a substrate. Panel A shows a representative experiment. (B) Quantification of the effect of 1 μg myelin on Histone H1-associated cdk5 kinase activity in cortical neurons. Data were obtained by PhosphorImager analysis of the auto-radiographs and are expressed as percentage of cdk5 activity found in neuronal cells grown on PDL. Average of four independent experiment ± SEM is shown, *P < 0.05 vs. PDL cultured neurons (Friedman Test followed by Dunn's Multiple Comparison Test). (C, D) Cortical neurons plated for 24 h on exercise-myelin display longer processes compared with cells plated on sedentary-myelin. Pictures of live neuronal cells were taken on a Zeiss inverted microscope (Scale bar = 50 μm)
Mini Protean Tgx Precast Protein Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mini protean tgx precast protein gel - by Bioz Stars, 2026-10
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polyclonal rabbit anti cdk5  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology polyclonal rabbit anti cdk5
<t>Cdk5</t> kinase activity is increased in primary cortical neurons grown on myelin extracted from the spinal cord of exercised animals. (A) Primary cortical neurons were grown for 24 h on PDL or on increasing concentration (0.5–2 μg) of myelin extracted from either sedentary (sed-myelin) or exercised animals (ex-myelin). Cdk5-associated kinase activity was determined after immunoprecipitation with anti-cdk5 antibodies and by using histone H1 as a substrate. Panel A shows a representative experiment. (B) Quantification of the effect of 1 μg myelin on Histone H1-associated cdk5 kinase activity in cortical neurons. Data were obtained by PhosphorImager analysis of the auto-radiographs and are expressed as percentage of cdk5 activity found in neuronal cells grown on PDL. Average of four independent experiment ± SEM is shown, *P < 0.05 vs. PDL cultured neurons (Friedman Test followed by Dunn's Multiple Comparison Test). (C, D) Cortical neurons plated for 24 h on exercise-myelin display longer processes compared with cells plated on sedentary-myelin. Pictures of live neuronal cells were taken on a Zeiss inverted microscope (Scale bar = 50 μm)
Polyclonal Rabbit Anti Cdk5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rgbs protein c388/90  (ImmunoGen Inc)
90
ImmunoGen Inc rgbs protein c388/90
<t>Cdk5</t> kinase activity is increased in primary cortical neurons grown on myelin extracted from the spinal cord of exercised animals. (A) Primary cortical neurons were grown for 24 h on PDL or on increasing concentration (0.5–2 μg) of myelin extracted from either sedentary (sed-myelin) or exercised animals (ex-myelin). Cdk5-associated kinase activity was determined after immunoprecipitation with anti-cdk5 antibodies and by using histone H1 as a substrate. Panel A shows a representative experiment. (B) Quantification of the effect of 1 μg myelin on Histone H1-associated cdk5 kinase activity in cortical neurons. Data were obtained by PhosphorImager analysis of the auto-radiographs and are expressed as percentage of cdk5 activity found in neuronal cells grown on PDL. Average of four independent experiment ± SEM is shown, *P < 0.05 vs. PDL cultured neurons (Friedman Test followed by Dunn's Multiple Comparison Test). (C, D) Cortical neurons plated for 24 h on exercise-myelin display longer processes compared with cells plated on sedentary-myelin. Pictures of live neuronal cells were taken on a Zeiss inverted microscope (Scale bar = 50 μm)
Rgbs Protein C388/90, supplied by ImmunoGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti ribosomal protein s6  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc mouse anti ribosomal protein s6
<t>Cdk5</t> kinase activity is increased in primary cortical neurons grown on myelin extracted from the spinal cord of exercised animals. (A) Primary cortical neurons were grown for 24 h on PDL or on increasing concentration (0.5–2 μg) of myelin extracted from either sedentary (sed-myelin) or exercised animals (ex-myelin). Cdk5-associated kinase activity was determined after immunoprecipitation with anti-cdk5 antibodies and by using histone H1 as a substrate. Panel A shows a representative experiment. (B) Quantification of the effect of 1 μg myelin on Histone H1-associated cdk5 kinase activity in cortical neurons. Data were obtained by PhosphorImager analysis of the auto-radiographs and are expressed as percentage of cdk5 activity found in neuronal cells grown on PDL. Average of four independent experiment ± SEM is shown, *P < 0.05 vs. PDL cultured neurons (Friedman Test followed by Dunn's Multiple Comparison Test). (C, D) Cortical neurons plated for 24 h on exercise-myelin display longer processes compared with cells plated on sedentary-myelin. Pictures of live neuronal cells were taken on a Zeiss inverted microscope (Scale bar = 50 μm)
Mouse Anti Ribosomal Protein S6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Cdk5 kinase activity is increased in primary cortical neurons grown on myelin extracted from the spinal cord of exercised animals. (A) Primary cortical neurons were grown for 24 h on PDL or on increasing concentration (0.5–2 μg) of myelin extracted from either sedentary (sed-myelin) or exercised animals (ex-myelin). Cdk5-associated kinase activity was determined after immunoprecipitation with anti-cdk5 antibodies and by using histone H1 as a substrate. Panel A shows a representative experiment. (B) Quantification of the effect of 1 μg myelin on Histone H1-associated cdk5 kinase activity in cortical neurons. Data were obtained by PhosphorImager analysis of the auto-radiographs and are expressed as percentage of cdk5 activity found in neuronal cells grown on PDL. Average of four independent experiment ± SEM is shown, *P < 0.05 vs. PDL cultured neurons (Friedman Test followed by Dunn's Multiple Comparison Test). (C, D) Cortical neurons plated for 24 h on exercise-myelin display longer processes compared with cells plated on sedentary-myelin. Pictures of live neuronal cells were taken on a Zeiss inverted microscope (Scale bar = 50 μm)

Journal:

Article Title: Exercise Decreases Myelin-Associated Glycoprotein Expression in the Spinal Cord and Positively Modulates Neuronal Growth

doi: 10.1002/glia.20521

Figure Lengend Snippet: Cdk5 kinase activity is increased in primary cortical neurons grown on myelin extracted from the spinal cord of exercised animals. (A) Primary cortical neurons were grown for 24 h on PDL or on increasing concentration (0.5–2 μg) of myelin extracted from either sedentary (sed-myelin) or exercised animals (ex-myelin). Cdk5-associated kinase activity was determined after immunoprecipitation with anti-cdk5 antibodies and by using histone H1 as a substrate. Panel A shows a representative experiment. (B) Quantification of the effect of 1 μg myelin on Histone H1-associated cdk5 kinase activity in cortical neurons. Data were obtained by PhosphorImager analysis of the auto-radiographs and are expressed as percentage of cdk5 activity found in neuronal cells grown on PDL. Average of four independent experiment ± SEM is shown, *P < 0.05 vs. PDL cultured neurons (Friedman Test followed by Dunn's Multiple Comparison Test). (C, D) Cortical neurons plated for 24 h on exercise-myelin display longer processes compared with cells plated on sedentary-myelin. Pictures of live neuronal cells were taken on a Zeiss inverted microscope (Scale bar = 50 μm)

Article Snippet: Lysates (50 μg) were immunoprecipitated with a saturating concentration of a rabbit polyclonal antibody against cdk5 (C8; Santa Cruz Biotechnology, Santa Cruz, CA) plus 20 μL of protein A-agarose (Santa Cruz Biotechnology) for 2 h at 4°C.

Techniques: Activity Assay, Concentration Assay, Immunoprecipitation, Cell Culture, Comparison, Inverted Microscopy

Cdk5-associated kinase activity is greatly elevated by exercise in the lumbar enlargement of rat spinal cord. (A) Exercise is a positive modulator of cdk5 kinase activity in the rat lumbar spinal cord region. The animals were exposed to exercise for 7 days. Cdk5 activity was determined after immunoprecipitation with anti-cdk5 antibodies and using histone H1 as a substrate. Data were obtained by PhosphorImager analysis of the autoradiographs. Data are mean ± SEM and are expressed as percentage of cdk5 activity measured in sedentary animals (*P < 0.05 (Student's t-test); n = 3 animals/group). Insert shows representative gel bands. (B) The increase in cdk5-associated kinase activity is proportional to the amount of volunteer running. The graph shows the correlation between individual variability in running and the increase in cdk5 activity (p = 0.0011; r = 0.973). Changes in kinase activity were plotted as function of the amount of running distance for each animal that ran for 7 days (open triangles) or remained sedentary (filled triangles).

Journal:

Article Title: Exercise Decreases Myelin-Associated Glycoprotein Expression in the Spinal Cord and Positively Modulates Neuronal Growth

doi: 10.1002/glia.20521

Figure Lengend Snippet: Cdk5-associated kinase activity is greatly elevated by exercise in the lumbar enlargement of rat spinal cord. (A) Exercise is a positive modulator of cdk5 kinase activity in the rat lumbar spinal cord region. The animals were exposed to exercise for 7 days. Cdk5 activity was determined after immunoprecipitation with anti-cdk5 antibodies and using histone H1 as a substrate. Data were obtained by PhosphorImager analysis of the autoradiographs. Data are mean ± SEM and are expressed as percentage of cdk5 activity measured in sedentary animals (*P < 0.05 (Student's t-test); n = 3 animals/group). Insert shows representative gel bands. (B) The increase in cdk5-associated kinase activity is proportional to the amount of volunteer running. The graph shows the correlation between individual variability in running and the increase in cdk5 activity (p = 0.0011; r = 0.973). Changes in kinase activity were plotted as function of the amount of running distance for each animal that ran for 7 days (open triangles) or remained sedentary (filled triangles).

Article Snippet: Lysates (50 μg) were immunoprecipitated with a saturating concentration of a rabbit polyclonal antibody against cdk5 (C8; Santa Cruz Biotechnology, Santa Cruz, CA) plus 20 μL of protein A-agarose (Santa Cruz Biotechnology) for 2 h at 4°C.

Techniques: Activity Assay, Immunoprecipitation